Title : Biosynthesis and characterization of silver nanoparticles from the Annona muricata leaves
Abstract:
Silver nanoparticles (AmL-AgNPs) were successfully synthesized using a green and eco-friendly approach by employing the leaf extract of Annona muricata. The formation and purity of these biogenic nanoparticles were initially confirmed through UV-vis spectroscopy and Energy Dispersive X-ray (EDX) analysis. A comprehensive characterization using various bio-physical techniques was conducted to understand the properties of the synthesized nanoparticles. Fourier Transform-Infrared (FT-IR) spectroscopic analysis revealed the presence of several organic functional groups, including alcohol, alkene, alkyl halide, amine, nitro, and sulfate, from the leaf extract acting as capping and stabilizing agents for the AmL-AgNPs. The morphology and structural details were examined using Field Emission Scanning Electron Microscopy (FESEM) and X-ray Diffraction (XRD). The FESEM analysis indicated the dense nature of the formed nanoparticles, while the XRD pattern confirmed their crystalline structure. The average crystalline size of the AmL-AgNPs was calculated from the XRD data and found to be 22.9 nm. Beyond characterization, the study evaluated the therapeutic potential of the synthesized nanoparticles. The AmL-AgNPs demonstrated significant biological activities, exhibiting potent anti-cancer properties against tested cell lines, notable anti-inflammatory effects, and promising anti-diabetic activity. In conclusion, this research successfully establishes a simple method for the synthesis of stable silver nanoparticles using A. muricata leaf extract. The significant multi-functional biological activities displayed by these nanoparticles highlight their potential as a versatile and multipurpose therapeutic agent, paving the way for further investigation into their pharmaceutical applications.
