Title : Photodynamic inactivation of antibiotic-resistant microflora using photosensitizer nanoforms
Abstract:
Purulent wounds treatment of soft tissues is associated with certain difficulties, which are caused by multiple factors, including occurrence, formation and wound nature specificities. Purulent complications significantly increase a treatment time and a process cost. We propose the problem solution of antibiotic-resistant microflora decontamination using technology based on fluorescent diagnostics and Photodynamic Inactivation (PDI). As nanocarriers, the photosensitizer ethosomes were used. Due to its neutral charge and spherical shape, the nanoparticles were able to penetrate through skin barrier and destroy pathogenic microorganisms.
70 patients were received PDI technology treatment of purulent wounds. Before and after irradiation, microflora composition and quantity were analyzed. The obtained data evidenced that local application of Photoran E6 and PDI technology inactivate both Gram-positive and Gram-negative bacteria. The use of photosensitizer nanocarriers led to obtain a quantitative decrease of bacterial growth in the wound of all patients and reduce the healing time of wounds.
PDI technology using application of photosensitizer nanocarriers showed high antimicrobial activity against antibiotic-resistant microflora of infected wounds in most clinical instances. The decontamination of microflora enabled to perform delayed reconstructive plastic surgery to close soft tissue defects and reduce hospitalization time of patients.
Audience Take Away:
- An improvement of patients treatment was the main aim of study. The obtained information could be used as a part technologists and doctors studying
- The results could help the audience in their job
- Other faculties to expand their researches or teachings could use this research
- This practical solution of the problem could simplify or make a designer’s job more efficient
