Title : Novel synthesis of quaternary Zn-Cu-In-S/ZnS QDs-m THPP porphyrin conjugate for photodynamic therapy and antibacterial activities
Abstract:
Photodynamic Therapy (PDT) is a non-invasive therapeutic approach that eliminates improperly developing cells and bacteria. Porphyrins are utilized in PDT as photosensitizers; nonetheless, their clinical applicability has been restricted due to their poor dispersity, which causes aggregation and low yields of reactive oxygen species. We present here the conjugation of (Zn)CuInS/ZnS quantum dots (QDs) to 5,10,15,20-tetrakis(3 hydroxyphenyl)porphyrin (mTHPP) to overcome these restrictions and boost PDT efficacy. The best circumstances for QDs-porphyrin conjugation production were thoroughly investigated. The bactericidal and PDT effectiveness of the produced (Zn)CuInS/ZnS - mTHPP conjugates were also evaluated in this study. The PDT effectiveness of mTHPP, QDs, and conjugate against the murine metastatic melanoma (B16 F10 Nex2) cell line was investigated with and without LED illumination. The conjugate had the greatest loss in cell viability after LED irradiation (72%) compared to the mTHPP (1%) and bare QDs (19%). Antimicrobial experiments on Escherichia coli (E. coli) revealed that the conjugation had a greater antibacterial impact than bare QDs, even in the absence of light. According to the findings, conjugate is a potential class of materials for anti-cancer and antibacterial PDT.
Audience take Away:
- Enhanced efficacy in Photodynamic Therapy (PDT) thus improving treatment outcomes in cancer and bacterial infections
- Simplification and optimization of PDT protocols
- Potential for reducing side effects and increasing patient comfort
- Expansion of knowledge in the fields of nanomedicine and antimicrobial treatments
- Basis for further research and development in related areas
- Integration into medical education and training programs
