Detection of graphene oxide in single HeLa cells based on MCR-Raman spectroscopy

Title : Detection of graphene oxide in single HeLa cells based on MCR-Raman spectroscopy

Abstract:

GO (Graphene Oxide) is a 2D nanomaterial that has attracted attention in many industries such as chemical, electronics and medical in recent years. Due to its unique properties such as strength, hydrophilicity and large specific surface area with the possibility of functionalization, GO is a particularly attractive material in biomedicine as a candidate for use in targeted drug delivery. The aim of this work is to study the presence of graphene oxide in cervical cancer cells by Raman spectroscopy, the fate of GO within a single cell based on mapping and its evaluation by MCR analysis. Raman spectroscopy proved to be a promising method that, together with MCR analysis, provided information on the detection of GO even in a single HeLa cell. Thus, GO can be used as a promising material with potential, for example, for drug delivery inside cancer cells, which can be further investigated for practical applications using Raman spectroscopy.

Audience Take Away:

• This work opens up the knowledge for the detection of GO in the cell using MCR-Raman spectroscopy.
• The MCR-Raman method offers a promising approach for the determination of GO, its degradation products or the interaction of GO with cellular components
• GO as a promising material with potential, for example, for drug delivery inside cancer cells.

Biography:

Zuzana Chaloupkova graduated in Biochemistry at Palacky University and in 2013. She received her MSc. In 2014 and joined the BioMed group led by Doc. Ranc in RCPTM (Regional Centre of Advanced Technologies and Materials) in Olomouc. In 2018, she has received her PhD degree in Physical Chemistry at the same institution. During her PhD study, she completed a 3-month internship at the University of Trieste under the supervision of Dr. Fornasaro. Since 2021 she became a junior researcher at CATRIN (Czech Advanced Technology and Research Institute) where she is still working. She is the author of 1 patent, first-author of 5 papers and co-author of other publications.