Preparation of Surface Modified Polymeric Nanoparticles of Doxorubicin

Title : Preparation of Surface Modified Polymeric Nanoparticles of Doxorubicin

Abstract:

Doxorubicin loaded PLGA nanoparticles were prepared and surface functionalization using trastuzumab, for targeted delivery. Nanoformulations were prepared by double emulsion solvent evaporation method using various stabilizers and size, PDI, zeta potential, entrapment efficiency was evaluated amount of drug loaded into the nanoparticles. The selected nanoformulations were surface functionalized by anti-HER2 antibody trastuzumab and SDS-PAGE analysis was applied to evaluate the activity and stability of antibody after surface modification. In-vitro release profile of selected nanoformulations exhibited biphasic release mechanism with moderate initial burst release followed by sustained release up to ten days. In-vivo pharmacokinetics of doxorubicin nanoparticles in comparison with reference drug ROBOL® in albino mice model exhibited significant increase in C max, AUC, AUMC, MRT and half life and decrease in volume of distribution and clearance was observed in comparison to reference drug. The in-vitro cellular uptake of these nanoformulations was studied usingy breast cancer cell line AU-565 and MCF-7. The results showed the cytotoxicity and IC50 factors were significantly improved in comparison with the control and without surface functionalized doxorubicin loaded PLGA nanoparticles. The prepared surface modified drug delivery system is designed to be administered through intravenous route for the better and improved results.

Biography:

Meritorious Professor Dr. Zafar Iqbal completed his B. Pharm. and M. Pharm. from Pakistan and then Ph.D. and Post Doc. in Pharmaceutical Sciences from University of Strathclyde, U.K. Then, joined production department in pharmaceutical industry followed by the teaching and research in the University of Peshawar. Now working as Dean of Life Sciences at Sarhad University of Science and Information Technology, Peshawar. He received Tamgha-e-Imtiaz, prestigious National Award, from the President of Pakistan. He has also been awarded Best University Teacher by HEC in 2007 and HEC Outstanding Research Paper Award 2011-2012. He established the laboratories and research facilities in the field of pharmaceutical sciences. Dr. Zafar Iqbal contributed to evaluate new DDIs, developed new drug-formulations with sustained release of drugs and on nano-pharmaceuticals. He supervised 31 Ph.D. and 12 M.Phil. Scholars and published more than 167 research articles. He is also co-author two chapters of the book published by Springer, New York. He served as a member of Central drug Licensing and Registration Board and currently is member of Drug Registration Board, Drug Expert Committee of Pakistan and Advance Studies and Research Boards of different universities. He has been awarded Best University Teacher by HEC in 2007 and HEC Outstanding Research Paper Award 2011-2012.